WebDigest your DNA: Set up restriction digests for your donor and recipient plasmids. Because you lose some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend 1.5-2μg of … WebCloneAmp™ HiFi PCR Premix Protocol-At-A-Glance (092612) www.clontech.com Clontech Laboratories, Inc. A Takara Bio Company Page 1 of 2 CloneAmp HiFi PCR Premix (Cat. …
CloneAmp™ HiFi PCR Premix Protocol-At-A-Glance - Takara Bio
WebCompared with the traditional enzyme ligation technology, the seamless cloning kit is simple and fast to operate, and is not limited by the restriction of the enzyme cleavage site. It can splicing multiple fragments at one time, which greatly improves the cloning efficiency. This Hieff Clone™ universal one step cloning kit is available for 1 ... WebHere's a list of top tips to keep in mind when designing your primers for seamless cloning, including some information specific to In-Fusion Cloning. The 3′ end of the primer should contain sequence that is specific to the target gene you are amplifying. This sequence should be 18–25 bases long and should ideally have a GC content between ... cumbernauld colts girls
一步法快速克隆试剂盒 Plus One Step Cloning Kit
Web23 de dez. de 2024 · To complement the bwd1 mutant, the coding region of AAE9 was induced into the pFGC-eYFP vector using the Hieff Clone Plus One Step Cloning Kit (Yeasen Biotechnology (Shanghai) Co. Ltd, Shanghai, China) driven by the 35S promoter to generate the 35S::AAE9-eYFP construct. Web655.00. 产品描述. Hieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒,精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反 … WebCompared with the traditional enzyme ligation technology, the seamless cloning kit is simple and fast to operate, and is not limited by the restriction of the enzyme cleavage site. It can splicing multiple fragments at one time, which greatly improves the cloning efficiency. This Hieff Clone™ universal one step cloning kit is available for 1 ... east towne center lancaster pa