Web14 de out. de 2024 · Luseogliflozin decreased HIF-1 target genes, such as the glucose transporter GLUT1 and the glycolytic enzymes HK2 and PKM, which have hypoxia … Web12 de mar. de 2024 · In silico analysis and chromatin immunoprecipitation assays demonstrated Nrf2 binding to the HIF1α promoter in normoxia, but its binding decreased …
肿瘤细胞代谢机制 - 豆丁网
WebThe kidney is particularly susceptible to ischemia/hypoxia insult while dysfunction of proximal tubular epithelial cells (PTEC) is a primary pathologic hallmark in acute kidney … All cell lines used were confirmed free from mycoplasma contamination, as determined by PCR. The human HCC cell lines HepG2 and Huh7 and lentiviral packaging line 293FT were grown in Dulbecco’s Modified Essential Media (DMEM, Hyclone), 10% fetal bovine serum (FBS, Atlanta Biologicals) or with … Ver mais Proliferation rates were assessed in cell lines by proliferation curve analysis and BrdU incorporation assays. For both assays, cells were plated on 6-cm plates in triplicate (50 × 103 … Ver mais Anchorage-independent growth assays were performed by plating 10–30 × 103cells in triplicate in 6-well plates in a mixture of media containing (0.35%) low-melt agarose over a … Ver mais To prepare samples, frozen cell pellets were lysed with lysis buffer: (20 mM) HEPES, (150 mM) NaCl, (1%) Triton X-100, (1 mM) EDTA, (1 mM) EGTA, (10 mM) sodium … Ver mais Cell death was assessed by either scoring apoptotic cells (cells with condensed or fragmented nuclei) visually at 20 × magnification after staining with the fluorescent DNA stain … Ver mais software v3400
The interplay between MYC and HIF in cancer - Nature
Web14 de mar. de 2012 · HIF-1 α is detected in the nucleus of proximal tubule cells after ischemia and in reperfusion (3-5-7days). Magnification: ×400 (b) Immunostaining for … WebNational Center for Biotechnology Information Web2 de out. de 2024 · HIF-2 is a member of heterodimeric transcription factors that are hypoxia-inducible factors. Similar to HIF-1, HIF-2 is composed of an alpha subunit and … software v22.3.4