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Pef6/v5-his b

WebContents 20 μg each pEF6/Myc-His A, B, and C, lyophilized 20 μg pEF6/Myc-His/lacZ, lyophilized Shipping/Storage Lyophilized plasmids are shipped at room temperature and … WebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia …

Shuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞

WebpEF6 V5-His TOPO® TA Vector Kit pEF6/V5-His TOPO® TA Vector Kit, included in the MembranePro™ Functional Protein Expression Kit and also available separately (see page 29), contains the reagents listed below. Upon receipt, store the vector kit at –20°C. Reagent Composition Amount pEF6/V5-His-TOPO® vector 10 ng/µL plasmid DNA 20 µL WebSep 29, 2014 · The expression plasmid pEF6/V5-His-Kif7 was generated by subcloning the Kif7 ORF from pCR-BluntII-TOPO-Kif7 (Imagenes) into pEF6/V5-His-TOPO (Life Technologies) using BstXI sites. The CCA cell lines JEG-3 and JAR were transfected with pEF6/V5-His-Kif7 or the control vector pEF6/V5-His using Lipofectamine 2000 (Life … how do geckos get their food https://carriefellart.com

Downregulation of the Gli Transcription Factors Regulator Kif7

WebVector Data. Vivid colors pcDNA6.2/C-EmGFP/GW/CAT . Restriction; Sequence; Map; Vector Data. pENTR L1-pLac-lacZalpha-L4 WebB I I Pme Comments for pEF6/V5-His A 5818 base pairs EF-1α promoter: bases 474-1651 T7 promoter/priming site: bases 1668-1687 Multiple cloning site: bases 1713-1804 V5 … WebApr 30, 2024 · PCR products were inserted into pEF6/V5-His B vector (Invitrogen) at EcoRV sites using In-Fusion HD Cloning Kit (TaKaRa) or NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA). The plasmids of dNlgns were modified by replacing V5-His tag with FLAG tag. Therefore, we obtained the following five plasmids: pEF6-dNlgn1-FLAG, … how much is holiday pay at amazon

pEF6 V5-His A Sequence and Map - SnapGene

Category:pEF6/V5-His A, B, and C - Thermo Fisher Scientific

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Pef6/v5-his b

ADAMTS4 and its proteolytic fragments ... - Wiley Online Library

WebShuffle T7 Express E.coli Strain大肠杆菌表达菌株及感受态细胞. 价 格: ¥0. 货 号: 3544763. 产 地: 北京. 点击询问 我要采购. 竭诚为您服务!. WebThe pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO™ Cloning. The pEF6/V5-His …

Pef6/v5-his b

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WebpEF6/V5-His (Search Vector Database) Backbone manufacturer Invitrogen Backbone size w/o insert (bp) 5840 Total vector size (bp) 6531 Vector type Mammalian Expression Selectable markers Blasticidin Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number Unknown … WebpEF6 V5-His B Sequence and Map Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pEF6/V5-His …

Webs871 streptavidin, rhodamine b 1 mg 1627.5. s872 streptavidin, texas red(r 1 mg 1627.5. s888 streptavidin 5 mg 2096.5. s911 streptavidin, horseradish 1 mg 1627.5. s921 streptavidin, alkaline ph 0.5 ml 2612.4. s931 streptavidin, beta-galact 1 mg 2144.8. s951 streptavidin agarose sed 5 ml 2128. sa10001 streptavidin - hrp 50ul 1449. sa10002 ...

WebDec 8, 2006 · HEK-293T cells were transfected with pEF6/V5-His recombinant vector containing Lrmp (A), Casc1 (B), Ghiso (C), or Lmna-rs1 (D) full-length cDNAs, grown for 48 h, fixed with paraformaldehyde, and treated with anti-V5 monoclonal antibody followed by secondary antibody-Alexa Fluor 488 conjugate. WebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia …

WebpEF6/V5-His A, B, and C - Gene Synthesis EN English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian český русский български العربية Unknown

WebpEF6-V5-His (Search Vector Database) Backbone size w/o insert (bp) 5840 Total vector size (bp) 8009 Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) … how do gears work on a bikeWebwere cloned separately into the pEF6 V5-His-TOPO mammalian expression vector (Invitrogen), and these plasmids were transfected into susceptible C3H He cells with Lipofectamine 2000 (Life Technologies, Grand Island, NY). Stable integrants were selected by using blasticidin S, and cells from individual foci were isolated with cloning rings and ... how do geese know when to fly southWebThe pEF6/V5-His-TOPO® expression vector and the monoclonal mouse anti-V5 antibody were purchased from Invitrogen. Genomic DNA isolated from peripheral blood lymphocytes of healthy European-, African-, Chinese-, and Hispanic-American volunteers was purchased from Coriell Cell Repositories (Camden, NJ). An enzymatic deglycosylation kit was ... how much is holiday pay in arizonaWebAll pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the … how much is holiday pay in albertahttp://www.biovector.net/product/44170.html how do geckos see the worldWebThe pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one … how do geese know where to flyWebInvitrogen™ pEF6/V5-His A, B, & C Mammalian Expression Vectors. Contains strong promoter for high-level expression in mammalian cells. Manufacturer: Invitrogen™ … how do geese survive the winter